At one event, Christians were referring to the Bible cities of Sodom and Gomorrah, which were destroyed due to the sexual behavior of the inhabitants who lived there.
Buttigieg is gay and openly talked about his episcopal faith. The presidential candidate of the Democratic Party tried to send disturbances without attacking the protesters, but admitted the memories and, at one point, the fact that men had the right to express their opinion.
"The good news is that the state of my soul is in the hands of God, but the Caucasus in Iowa depends on you," Butigig said.
Interruptions to political events are frequent during the campaign. Former Boto representative O'Rourke, Sen Curry Booker and Bernie Sanders all these protests shouted this year. But the anti-gay character of the songs in Butig on Tuesday hampered personal injuries.
Anti-gay feelings have led Iowa's policy for years, especially with some vocal social conservative groups, but the country was among the first to legalize the same marriage 10 years ago, something Butigig admitted on Tuesday. It has long been a hot button topic in Des Moines, where several gay democrats are elected to local and state offices.
But Butigigan demonstrators, led by an anti-abortion activist, Randall Terry, came out of the country to attack the presidential candidate. Terry was spotted driving a large RV past the event after the event.
After the protest, Terry said he also broke the Booker event earlier on Tuesday, but that he planned to attend as many Butigig events as possible.
The first disorder came in Fort Dodge, Iowa, where a man at the 100-member event stopped and shouted: "We will not stop, he misleads our children."
The man was summoned by the audience and after being removed from the event, Buttigieg said: "We are so dug in such a passionate way."
"This gentleman believes that what he does is in line with the creator's will," he said. "I look differently, we need to be able to see it differently."
The two demonstrators interrupted the next show of Butigger in De Moine by shouting "Remember Sodom and Gomorrah" at different times during the event.
Re-singing refers to two Bible cities where, according to the Bible book of Judah, the inhabitants "surrendered to sexual immorality" and, as punishment, "will serve as an example by punishing eternal fire."
They both shouted for a long time, but they could not hear the songs from the audience.
Buttigieg told CNN on Tuesday that "he would prefer such debates in a respected format, as opposed to a break, but the president will have to tackle the more stringent things rather than be interrupted by the speech."
"That's what it is," he said as he spoke to the voters after the event.
The mayor added that he did not receive much during his time in the South Bend.
"We are all from the same community, so maybe it's a little different. There were some crap, there will always be," he said. "I have the feeling that people here believe in a respected debate. We can have a respectable debate all day, but ideally without interrupting one another."
The voter asked Chasten Butigig, the husband of the candidate, and for hecklers after the event.
"We are out of partition," he said. "That's what we're here to talk about."
Former little known Butighget, following a well-cited CNN headquarters in March, saw a wave of momentum in his presidential campaign, leading to his official announcement Sunday in the South Bend.
The mayor made his sexuality, his marriage and his religion part of his turn towards the voters.
Buttigieg came out in 2015, months before being re-elected mayor of South Bend. He talked about the fact that he was uncomfortable when he was gay early in his life and told the audience at the LGBTK event earlier this month that "if they showed me exactly what it was that made me gay, I would cut it with a knife."
He later added: "Thank God, there was no pill. Thank God that there was no knife."
CNN Vanessa Jurkevich and Aaron Cooper contributed to this report.