The motion brought against Mashaba by the ANC will be debated in council on Thursday.
Mashaba has declined to comment on the matter.
DA Johannesburg regional chairperson Tsepo Mhlongo said on Monday that since Johannesburg residents had seen the ANC's poor service delivery and corruption laws and had begun to witness Mashaba's changes, they were desperate to halt its progress.
"Under his leadership, the DA-led city of Johannesburg consistently delivers on its three broad election commitments of job creation, delivering quality services and rooting out corruption," Mhlongo said.
Mhlongo said the city's financial strength was better than ever before, citing ANC citations as grounds for its motion.
He said the metro spent more than 93% of its capital budget and more than 99% of its infrastructure grant for two consecutive years.
“These are the changes residents have waited for decades to see. Mayor Mashaba and his team have achieved more in three years than the ANC was able to accomplish in more than 20 years.
“With the DA at the helm, the people of Joburg will always come first.
"This will not be the case under ANC administration."
The party called on the people of Johannesburg to sign a petition in support of the mayor on their website: www.handsoffmashaba.co.za.